Choosing blood collection tubes mainly depends on whether you want to collect serum or plasma. Serum is the product of blood that
has been allowed to clot. Plasma is the product of blood that has been prevented from clotting with an anticoagulant,while serum tubes
contain silica nanoparticles or thrombin to induce clotting. When using plasma tubes, check that the anticoagulant is compatible with
your down stream experiments.
In this short article, we explain different types of blood collection tube, including plasmatubes, serum tubes, and their key differences.
Blood Collection Tube Types
The first thing to figure out is whether you want to collectserum or plasma. That depends on whether or not you need to stop the blood
from clotting.
There are two main types of serum collection tubes that differ in how they activate blood clotting, using different clot activators.
These tubes have silica nanoparticles, which activate clotting by activating coagulation factor XII. Some also have a gel to separate the
serum.Those without the separating gel are usually more useful in sensitive diagnostic testing.
Color of the cap: Red
Clotting time: Approximately30 minutes.
These tubes use thrombin to activate blood clotting. They’remainly used clinically for tests that are needed especially quickly. However, some serum components are a little less stable in these tubes.
Color of the cap: Orange.
Clotting time: Approximately 5 minutes.
This is the category to consider if you need cells or plasma (acell-free liquid that still contains coagulation factors).
Contains EDTA anticoagulant, in the routine blood test, EDTA anticoagulant compared with other anticoagulants, the coagulation of
bloodcells and the morphology of blood cells is less affected, so usually use EDTA salt (2K, 3K, 2Na) as anticoagulant. Note that
these tubes containeither K2EDTA (dipotassium) or K3EDTA (tripotassium). Beware that K3EDTA ismore hyperosmolar due to
the extra potassium ion—meaning it will concentrate blood samples more than K2EDTA via the hyperosmolar effect.
For coagulation and platelet function tests. Like EDTA, citrateremoves calcium from the blood by chelating it. Unlike EDTA, it’s reversible—socalcium can be added back to study coagulation under controlled conditions.Citrated plasma is also used to measure coagulation-relevant factors.
Also, note that different citrate concentrations are availablefrom different companies, so check this before you buy them.
Heparin is added to the blood collection tube. Heparin has the effect of antithrombin directly, which can prolong the clotting time of the
specimen, and excessive heparin can cause the aggregation of leukocytes and cannot be used for leukocyte counting.
For more specific purposes, there are additional types of blood collection tubes out there, but hopefully, this has given you a handle on
where to start. Remember to consider whether you want plasma or serum, and pick your tube accordingly.
Note: Please remember to always wear protective equipment when working with blood, including safety goggles, gloves, and laboratory
coats, andwork carefully to avoid contact with blood and blood exposure. It’s important to consider the safety of yourself and others in
the lab.
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